Rarg Polyclonal Antibody for Mouse(Mus musculus) | EIAab.comRarg Polyclonal Antibody for Mouse(Mus musculus)

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Rarg (Gene name),

Retinoic acid receptor gamma (Protein name ), RARG_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Rarg(Nr1b3);

Protein name:

Retinoic acid receptor gamma(RAR-gamma);

Alternative:

Nuclear receptor subfamily 1 group B member 3;

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Homodimer (By similarity). Heterodimer with a RXR molecule (By similarity). Binds DNA preferentially as a RAR/RXR heterodimer (By similarity). Forms a complex with PUS1 and the SRA1 RNA in the nucleus.

Function:

Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, acts mainly as an activator of gene expression due to weak binding to corepressors (By similarity). Required for limb bud development. In concert with RARA or RARB, required for skeletal growth, matrix homeostasis and growth plate function.

Subcellular Location:

Nucleus

Protein Attributes:

Sequence length:

458

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

458:

SPQPDQGP

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

P18911

Pfam:

PF00104

UniGene:

Mm.1273

KEGG:

mmu:19411

SMR:

P18911

Uniprot:

P18911

FOR

ELISA Kit for Mouse RAR-gamma

Cat.:

E10643h

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Packing:

96T

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ELISA Kit for Mouse RAR-gamma

Cat.:

E10643m

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96T

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CLIA Kit for Mouse RAR-gamma

Cat.:

U10643m

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96T

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CLIA Kit for Mouse RAR-gamma

Cat.:

U10643h

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MSDS:

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Packing:

96T

Polyclonal Antibody for Mouse RAR-gamma

Cat.:

P10643Rb-m

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Mouse RAR-gamma

Cat.:

P10643Rb-h

Price:

Please sign in first.

Packing:

40ug/0.2ml

Monoclonal Antibody for Mouse RAR-gamma

Protein for Mouse RAR-gamma

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.1%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of RARG into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	95	91-103
Plasma	97	93-104

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RARG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-99%	90-102%	88-103%	92-98%
EDTA plasma(n=5)	89-106%	93-100%	90-105%	92-101%
heparin plasma(n=5)	93-103%	94-102%	91-103%	95-100%

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Cloning of murine alpha and beta retinoic acid receptors and a novel receptor gamma predominantly expressed in skin."
Zelent A. , Krust A. , Petkovich M. , Kastner P. , Chambon P.
Nature339:714-717(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);TISSUE SPECIFICITY
2.

"Identification of a novel isoform of the retinoic acid receptor gamma expressed in the mouse embryo."
Giguere V. , Shago M. , Zirngibl R. , Tate P. , Rossant J. , Varmuza S.
Mol. Cell. Biol.10:2335-2340(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2)
3.

"The transcriptional landscape of the mammalian genome."
Carninci P. , Kasukawa T. , Katayama S. , Gough J. , Frith M.C. , Maeda N. , Oyama R. , Ravasi T. , Lenhard B. , Wells C. , Kodzius R. , Shimokawa K. , Bajic V.B. , Brenner S.E. , Batalov S. , Forrest A.R. , Zavolan M. , Davis M.J. , more...
Science309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
strain: C57BL/6J.
4.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
strain: Czech II.
tissue: Mammary tumor.
5.

"Murine isoforms of retinoic acid receptor gamma with specific patterns of expression."
Kastner P. , Krust A. , Mendelsohn C. , Garnier J.-M. , Zelent A. , Leroy P. , Staub A. , Chambon P.
Proc. Natl. Acad. Sci. U.S.A.87:2700-2704(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-74 (ISOFORMS 1; 2 AND 3)
6.

"Function of retinoic acid receptor gamma in the mouse."
Lohnes D. , Kastner P. , Dierich A. , Mark M. , LeMeur M. , Chambon P.
Cell73:643-658(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISRUPTION PHENOTYPE;FUNCTION
7.

"Regulation of nuclear receptor activity by a pseudouridine synthase through posttranscriptional modification of steroid receptor RNA activator."
Zhao X. , Patton J.R. , Davis S.L. , Florence B. , Ames S.J. , Spanjaard R.A.
Mol. Cell15:549-558(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION IN A COMPLEX WITH PUS1 AND SRA1;SUBCELLULAR LOCATION
8.

"Retinoic acid receptors are required for skeletal growth, matrix homeostasis and growth plate function in postnatal mouse."
Williams J.A. , Kondo N. , Okabe T. , Takeshita N. , Pilchak D.M. , Koyama E. , Ochiai T. , Jensen D. , Chu M.L. , Kane M.A. , Napoli J.L. , Enomoto-Iwamoto M. , Ghyselinck N. , Chambon P. , Pacifici M. , Iwamoto M.
Dev. Biol.328:315-327(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISRUPTION PHENOTYPE;FUNCTION
9.

"Analysis of Cyp26b1/Rarg compound-null mice reveals two genetically separable effects of retinoic acid on limb outgrowth."
Pennimpede T. , Cameron D.A. , MacLean G.A. , Petkovich M.
Dev. Biol.339:179-186(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISRUPTION PHENOTYPE;POSSIBLE FUNCTION;DEVELOPMENTAL STAGE

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